Topic outline

  • New parameters of hematology analyzers and their clinical significance

    Speaker: Johannes (Hans) Hoffmann (DE)
    Moderator: Giuseppe Lippi (IT)

    Date: 12th February 2019 at 18:00 CET


    Modern hematology analyzers do much more than just counting blood cells. Practically all instruments currently available also perform a white blood cell (WBC) differential and they produce flags or alerts for possible morphological abnormalities. Hematology analyzers have completely replaced traditional methods of cell counting and microscopic blood smear examination for the vast majority of blood samples.

    In addition to these capabilities, hematology analyzers generate an ever-increasing amount of raw data, which can be transformed into cell parameters, for which no historical equivalent exist. This trend already started in the early days when measuring and reporting mean red cell volume (MCV) became possible. MCV has evidently established its clinical significance, due to the crucial role in classifying anemia. After MCV, many other parameters have been developed, not only for red blood cells, but also for platelets and white blood cells. However, in contrast to MCV, the clinical relevance of many of the new parameters is still unclear. Hematology analyzer manufacturers are generally launching these new parameters as research tools, without the slightest clue whether they can be of clinical value. This then opens possibilities for laboratory professionals examining the potential clinical significance of such parameters. Unfortunately, this type of research is plagued by serious challenges: most of the new parameters are not internationally standardized, limiting their widespread use in clinical practice. Sometimes the parameters depend on a certain technology, meaning that only laboratories using a specific make of hematology analyzer can use the parameter. And last but not least, some parameters are even specific for one individual hematology analyzer; other analyzers of the same model may produce different results and thus require separate clinical validation. An example of the latter are the so-called cell population data that have attracted a lot of interest in recent years; some interesting and promising applications were described, but one should also be aware of the limitations of this type of parameters.

    The primary aim of this presentation is to discuss the technological principles used by the major hematology analyzers and to demonstrate how these technologies generate new cellular parameters. The second part will focus on understanding the potential use and limitations of new parameters, which are essential for researchers who are interested in investigating their clinical application. 

    About the speaker

    Johannes HoffmannDr. Johannes (Hans) Hoffmann was born in 1952 in Rotterdam, the Netherlands. He started his career in clinical chemistry and laboratory medicine in 1976 as a trainee. Once certified as a specialist he became the head of the hematology laboratory in a large tertiary-care teaching hospital in the Netherlands, where few years later he was also appointed chairman of the Department of Clinical Laboratories. In 1992 he obtained his PhD in medical sciences at Leiden University, the Netherlands, on a thesis in the field of fibrinolysis. He is EFLM-certified as a specialist in laboratory medicine with hematology as a focus.

    After more than 25 year in this hospital position, Hans moved in 2008 to Abbott Diagnostics, where he was responsible for global scientific affairs in hematology. Apart from conducting scientific studies, he was involved in the development process of Abbott’s new hematology analyzer, particularly for supporting the algorithms that convert the primary measurement data into numerical and graphical information that is presented to the end user of the analyzer. In that position he was also a member of several international committees and working groups on standardization in laboratory hematology.

    Hans concluded his active career in late 2017. He is still a reviewer for various journals in the field of laboratory medicine and hematology. His scientific work comprises over 120 papers in peer-reviewed journals, mainly focused on general hematology, flow cytometry, coagulation and fibrinolysis. He is also author and co-author of textbooks on laboratory medicine and hematology.

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